Publications

  1. Albert Kamanzi, Ariadne Tuckmantel Bido, Yao Zhang, Erik Olsén, Maya Stibbards-Lyle, Martin Jasinski, Yifei Gu, Benjamin Wang, Michael Venier-Karzis, Romain Berti, Michelle Jeliazkova, Cynthia Shaheen, Jerry Leung, Miffy Cheng, Pieter Cullis, Sabrina Leslie. “Single-particle multi-parametric microscopy reveals structural, size, and payload heterogeneity in mRNA-loaded lipid nanoparticles”. Submitted and posted on BiorXiv.
  2. Frank Stabile, Cynthia Shaheen, Sabrina Leslie. “New frontiers in applied biophysics: advancing drug discovery using single-molecule microscopy”. Submitted review article (2025).
  3. Cynthia Shaheen, Bianca Caminada, Alexis Hilts, Ruby Wei, Jay Botham, Rebecca Johnson, Nane Vardanyan, Eric Boateng, Sabrina Leslie. “Stemless molecular beacon probes for single-molecule detection of supercoil-induced DNA denaturation.” In review. https://www.researchsquare.com/article/rs-7377411/v1
  4. E. Boateng, E. Olsen, A. Kamanzi, Y. Zhang, B. Zhao, P. Cullis, S. Leslie. “Multiparametric Characterization of Individual Suspended Nanoparticles using Confocal Fluorescence and Interferometric Scattering Microscopy with Microfluidic Confinement.” Nano Letters 2025. https://doi.org/10.1021/acs.nanolett.5c02232
  5. Yulin Mo, Alexander, F. A. Keszei, Shagun Kothari, Heyi Liu, Anni Pan, Paige Kim, Jiachuan Bu, Albert Kamanzi, David L. Dai, Mohammad T. Mazhab-Jafari, Juan Chen, Sabrina Leslie and Gang Zheng. “Lipid-siRNA Organization Modulates the Intracellular Dynamics of Lipid Nanoparticles.” J. Am. Chem. Soc. 2025, 147, 12, 10430–10445. https://doi.org/10.1021/jacs.4c18308
  6. Micaela Belleperche, Jiawen Liu, Yuhao Chen, Chuyang Zhang, Kimiya Karimi, Sabrina Leslie, Maureen McKeague. “A Generalizable Screening Platform for Developing Functional Aptasensors”. Analytical Chemistry 2025, 97, 14, 7643–7650. https://doi.org/10.1021/acs.analchem.4c04120
  7. Albert Kamanzi, Yao Zhang, Yifei Gu, Faith Liu, Romain Berti, Benjamin Wang, Fariba Saadati, Marco A. Ciufolini, Jayesh Kulkarni, Pieter Cullis, and Sabrina Leslie. “Quantitative Visualization of Lipid Nanoparticle Fusion as a Function of Formulation and Process Parameters.” ACS Nano 2024 18 (28), 18191-18201. https://doi.org/10.1021/acsnano.3c12981
  8. Bin Zhao, Albert Kamanzi, Yao Zhang, Karen Y.T. Chan, Madelaine Robertson, Sabrina Leslie, Pieter R. Cullis. “Determination of the interior pH of lipid nanoparticles using a pH-sensitive fluorescent dye-based DNA probe”. Biosensors and Bioelectronics, Volume 251, 1 May 2024, 116065. https://doi.org/10.1016/j.bios.2024.116065
  9. C. Shaheen, C. Hastie, K. Metera, S. Scott, Z. Zhang, S. Chen, G. Gu, L. Weber, B. Munsky, D. Levens, C. Benham, and S. R. Leslie. “Non-equilibrium structural dynamics of supercoiled DNA plasmids exhibit asymmetrical relaxation.” Nucleic Acids Research (2022), 50(5): 2754-2764. https://doi.org/10.1093/nar/gkac101
  10. A. Kamanzi, Y. Gu, R. Tahvildari, Z. Friedenberger, X. Zhu, R. Berti, M. Kurylowicz, D. Witzigmann, J. Kulkarni, J. Leung, John Andersson, Andreas Dahlin, Fredrik Hook, Mark Sutton, Pieter R. Cullis, and S. R. Leslie. “Simultaneous, single-particle measurements of size and loading give new insights into the structure of drug-delivery nanoparticles.” ACS Nano (2021), 15, 12, 19244-19255. https://doi.org/10.1021/acsnano.1c04862
  11. S. R. Leslie, et. al. “Single-molecule imaging of the biophysics of molecular interactions in cell-like conditions, without tethers, and with precision and control.” Current Opinion in Biomedical Engineering (2019) 12: 75-82. https://doi.org/10.1016/j.cobme.2019.10.004
  12. S. Scott, C. Shaheen, B. McGuiness, K. Metera, F. Kouzine, D. Levens, C. J. Benham, S. R. Leslie. “Single-molecule visualization of the effects of ionic strength and crowding on structure-mediated interactions in supercoiled DNA molecules.” Nucleic Acids Research (2019), 47, 12, 6360-6368. https://doi.org/10.1093/nar/gkz408
  13. M. Shayegan, R. Tahvildari, L. Kisley, K. Metera, S. W. Michnick, S. R. Leslie. “Probing inhomogeneous diffusion in the microenvironments of phase-separated polymers under confinement.” Journal of the American Chemical Society. (2019), 141, 19, 7751-7757. https://doi.org/10.1021/jacs.8b13349
  14. K. Thiombane, N. Coutin, D. J. Berard, R. Tahvildari, S. R. Leslie, C. Nislow. “Single-cell analysis for drug development using Convex Lens-Induced Confinement (CLiC) imaging.” BioTechniques (2019) 67(5): 210-217. https://doi.org/10.2144/btn-2019-0067
  15. D. J. Berard, S.R. Leslie. “Miniaturized flow cell with pneumatically-actuated vertical nanoconfinement for single-molecule imaging and manipulation.” Biomicrofluidics (2018), 12, 054107. https://doi.org/10.1063/1.5052005
  16. S. Scott, et. al. “Visualizing structure-mediated interactions in supercoiled DNA molecules” Nucleic Acids Research. (2018), 10, 1093. https://doi.org/10.1093/nar/gky266
  17. T. Shendruk, D. Sean, D. Berard, J. Wolf, J. Dragoman, S. Battat, G. Slater, S. R. Leslie. “Rotation-induced Macromolecular Spooling DNA” Physical Review X 7, (2017), 031005(1-11).
    https://doi.org/10.48550/arXiv.1706.01861
  18. D. Berard, M. Shayegan, F. Michaud, G. Henkin, S. Scott, S. R. Leslie. “Formatting and Ligating Biopolymers using Adjustable Nanoconfinement.” Applied Physics Letters (2016), 109, 033702. https://doi.org/10.1063/1.4958196
  19. G. Henkin, D. Berard, F. Stabile, M. Shayegan, J. S. Leith, S. R. Leslie. “Manipulating and visualizing molecular interactions in customized nanoscale spaces.” Analytical Chemistry (2016) 88(22), 11100-11107. https://doi.org/10.1021/acs.analchem.6b03149
  20. J. S. Leith, A. Kamanzi, D. Sean, D. Berard, A. Guthrie, C.M.J. McFaul, G. Slater, H. de Haan, S. R. Leslie. “Free energy of a polymer in slit-like confinement from the Odijk to the bulk regime.” Macromolecules (2016), 49 (23) 9266-9271. https://doi.org/10.1021/acs.macromol.6b01805
  21. B. Jia, T.L. Wee, D. Berard, A. Mallik, D. Juncker, C. M. Brown, S. R. Leslie. “Parallelized Cytoindentation Using Convex Micropatterned Surfaces.” Biotechniques (2016), 61, (2), 73-82. https://doi.org/10.2144/000114436
  22. J. Ahamed, S. Mahshid, D. Berard, F. Michaud, R. Sladek, W. Reisner, S. R. Leslie. “Continuous Confinement Fluidics: Getting Lots of Molecules in Small Spaces.” Macromolecules (2015), 49, (7) 2853-2859. https://doi.org/10.1021/acs.macromol.5b02617
  23. S. Mahshid, M. J. Ahamed, D. Berard, S. Amin, R. Sladek, S. R. Leslie, W. Reisner. “Development of a Platform for Single-Cell Genomics Using Convex Lens-Induced Confinement.” Lab on a Chip (2015), 15, 3013-3020. https://doi.org/10.1039/C5LC00492F
  24. A. Arsenault, J. S. Leith, G. Henkin, C. McFaul, M. Tarling, R. Talbot, D. Berard, F. Michaud, S. Scott, S. R. Leslie. “Open-frame System for Single–Molecule Microscopy.” Review of Scientific Instruments (2015), 86 (3), 033701. https://doi.org/10.1063/1.4913271
  25. D. Berard, F. Michaud, S. Mahshid, M. J. Ahamed, C. McFaul, J. S. Leith, P. Berube, R. Sladek, W. Reisner, S. R. Leslie. “Convex lens-induced nanoscale templating.” P.N.A.S. (2014), 111, 37. https://doi.org/10.1073/pnas.1321089111
  26. D. Berard, C. McFaul, J. S. Leith, A. Arsenault, F. Michaud, S. R. Leslie. “Precision Platform for Convex Lens-Induced Confinement Microscopy.” Review of Scientific Instruments (2013), 84, 103704. https://doi.org/10.1063/1.4822276
  27. M. W. Elting, S. R. Leslie, L. S. Churchman, J. Korlach, C. McFaul, J. S. Leith, M. J. Levene, A. E. Cohen, J. A. Spudich. “Single- molecule fluorescence imaging of processive myosin with enhanced background suppression using linear Zero Mode Waveguides (ZMW) and Convex Lens-induced Confinement (CLiC).” Optics Express (2013), 21 (1), 1189-1202. https://doi.org/10.1364/OE.21.001189
  28. S. R. Leslie, A. P. Fields, A. E. Cohen. “Convex Lens-induced Confinement for Imaging Single Molecules.” Analytical Chemistry (2010) 82 (14), 6224-6229. https://doi.org/10.1021/ac101041s
  29. A. E. Cohen, A. P. Fields, J. H. Hou, S. R. Leslie, M. J. Shon. “In honor of W. E. Moerner: Confining molecules for single-molecule spectroscopy.” Israel Journal of Chemistry (2010) 49 (3-4), 275. https://doi.org/10.1560/IJC.49.3-4.275
  30. J. D. Sau, S.R. Leslie, M. L. Cohen, D.M. Stamper-Kurn. “Spin squeezing of high-spin, spatially extended quantum fields.” New Journal of Physics (2010) 12, 085011.
    https://doi.org/10.48550/arXiv.1005.0603
  31. M. Vengalattore, J. Guzman, S. R. Leslie, F. Serwane, and D. M. Stamper-Kurn. “Periodic spin textures in a degenerate F=187Rb spinor Bose gas.” Physical Review A (2010) 81, 053612. https://doi.org/10.48550/arXiv.0901.3800
  32. S. R. Leslie, J. Guzman, M. Vengalattore, J.D. Sau, M.L. Cohen, D.M. Stamper-Kurn. “Amplification of fluctuations in a spinor Bose Einstein condensate.” Physical Review A (2009) 79, 043631. https://doi.org/10.1103/PhysRevA.79.043631
  33. J. D. Sau, S.R. Leslie, D.M. Stamper-Kurn, M.L. Cohen. “Theory of domain formation in inhomogeneous ferromagnetic dipolar condensates.” Physical Review A (2009) 80, 023622. https://doi.org/10.1103/PhysRevA.80.023622
  34. M. Vengalattore, S.R. Leslie, J. Guzman, D.M. Stamper-Kurn. “Spontaneously modulated spin textures in a dipolar spinor Bose-Einstein condensate.” Physical Review Letters (2008) 100, 170403. https://doi.org/10.1103/PhysRevLett.100.170403
  35. M. Vengalattore, J. M. Higbie, S. R. Leslie, J. Guzman, L. E. Sadler, D. M. Stamper-Kurn. “High-resolution magnetometry with a spinor Bose-Einstein condensate.” Physical Review Letters (2007) 98, 200801. https://doi.org/10.1103/PhysRevLett.98.200801
  36. L. E. Sadler, J. M. Higbie, S. R. Leslie, M. Vengalattore, D. M. Stamper-Kurn. “Coherence-enhanced imaging of a degenerate Bose gas.” Physical Review Letters (2007) 98, 110401. https://doi.org/10.1103/PhysRevLett.98.110401
  37. L. E. Sadler, J. M. Higbie, S. R. Leslie, M. Vengalattore, D. M. Stamper-Kurn. “Spontaneous symmetry breaking in a quenched ferromagnetic spinor Bose condensate.” Nature (2006) 443, 312. https://doi.org/10.48550/arXiv.cond-mat/0605351
  38. J. M. Higbie, L. E. Sadler, S. Inouye, A. P. Chikkatur, S. R. Leslie, K. L. Moore, V. Savalli, D. M. Stamper-Kurn. “Direct, non-destructive imaging of magnetization in a spin-1 Bose gas.” Physical Review Letters (2005) 95, 050401. https://doi.org/10.1103/PhysRevLett.95.050401
  39. K. L. Moore, T. P. Purdy, K. W. Murch, S. Leslie, S. Gupta, and D. M. Stamper-Kurn. “Collimated, single-pass atom source from a pulsed alkali metal dispenser for laser-cooling experiments.” Review of Scientific Instruments (2005) 76, 023106. https://doi.org/10.1063/1.1841852
  40. S. Leslie, N. Shenvi, K. R. Brown, Dan M. Stamper-Kurn, and K. Birgitta Whaley. “Transmission spectrum of an optical cavity containing N atoms.” Physical Review A (2004) 69, 043805. https://doi.org/10.1103/PhysRevA.69.043805